CXCL1 promotes colon cancer progression through activation of NF-κB/P300 signaling pathway.

ABSTRACT:

Background

The upregulated expression of CXCL1 has been validated in colorectal cancer patients. As a potential biotherapeutic target for colorectal cancer, the mechanism by which CXCL1 affects the development of colorectal cancer is not clear.

Methods

Expression data of CXCL1 in colorectal cancer were obtained from the GEO database and verified using the GEPIA database and the TIMER 2.0 database. Knockout and overexpression of CXCL1 in colorectal cancer cells by CRISPR/Cas and "Sleeping Beauty" transposon-mediated gene editing techniques. Cell biological function was demonstrated by CCK-8, transwell chamber and Colony formation assay. RT-qPCR and Western Blot assays measured RNA and protein expression. Protein localization and expression were measured by immunohistochemistr

SUBMITTER: Zhuo C 

PROVIDER: S-EPMC9701058 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature