A pipeline for malignancy and therapy agnostic assessment of cancer drug response using cell mass measurements.

ABSTRACT: Functional precision medicine offers a promising complement to genomics-based cancer therapy guidance by testing drug efficacy directly on a patient's tumor cells. Here, we describe a workflow that utilizes single-cell mass measurements with inline brightfield imaging and machine-learning based image classification to broaden the clinical utility of such functional testing for cancer. Using these image-curated mass measurements, we characterize mass response signals for 60 different drugs with various mechanisms of action across twelve different cell types, demonstrating an improved ability to detect response for several slow acting drugs as compared with standard cell viability assays. Furthermore, we use this workflow to assess drug responses for various primary tumor specimen formats in

SUBMITTER: Kimmerling RJ 

PROVIDER: S-EPMC9701192 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature