Rad52's DNA annealing activity drives template switching associated with restarted DNA replication.

ABSTRACT: It is thought that many of the simple and complex genomic rearrangements associated with congenital diseases and cancers stem from mistakes made during the restart of collapsed replication forks by recombination enzymes. It is hypothesised that this recombination-mediated restart process transitions from a relatively accurate initiation phase to a less accurate elongation phase characterised by extensive template switching between homologous, homeologous and microhomologous DNA sequences. Using an experimental system in fission yeast, where fork collapse is triggered by a site-specific replication barrier, we show that ectopic recombination, associated with the initiation of recombination-dependent replication (RDR), is driven mainly by the Rad51 recombinase, whereas template switching, du

SUBMITTER: Kishkevich A 

PROVIDER: S-EPMC9701231 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature