Candidate genes and their alternative splicing may be potential biomarkers of acute myocardial infarction: a study of mouse model.

ABSTRACT:

Background

Acute myocardial infarction (AMI) is one of the leading causes of death in human being, and an effective diagnostic biomarker is still lacking. Whilst some gene association with AMI has been identified by RNA sequencing (RNA-seq), the relationship between alternative splicing and AMI is not clear.

Methods

We retrieved myocardial tissues within 24 h from mice with induced AMI and sham, and analysed the differentially expressed genes (DEGs) and differential alternative splicing genes (DASGs) by RNA-seq. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and protein interaction network analysis were performed on DEGs-DASGs-overlap genes. PCR was used to verify the expression levels of representative genes and alternative splicing in my

SUBMITTER: Liu X 

PROVIDER: S-EPMC9701406 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature