Development of a flow standard to enable highly reproducible measurements of deformability of stored red blood cells in a microfluidic device.

ABSTRACT:

Background

Great deformability allows red blood cells (RBCs) to flow through narrow capillaries in tissues. A number of microfluidic devices with capillary-like microchannels have been developed to monitor storage-related impairment of RBC deformability during blood banking operations. This proof-of-concept study describes a new method to standardize and improve reproducibility of the RBC deformability measurements using one of these devices.

Study design and methods

The rate of RBC flow through the microfluidic capillary network of the microvascular analyzer (MVA) device made of polydimethylsiloxane was measured to assess RBC deformability. A suspension of microbeads in a solution of glycerol in phosphate-buffered saline was developed to be used as an internal flow rate ref

SUBMITTER: Robidoux J 

PROVIDER: S-EPMC9701565 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature