Alterations induced by the PML-RARα oncogene revealed by image cross correlation spectroscopy.

ABSTRACT: The molecular mechanisms that underlie oncogene-induced genomic damage are still poorly understood. To understand how oncogenes affect chromatin architecture, it is important to visualize fundamental processes such as DNA replication and transcription in intact nuclei and quantify the alterations of their spatiotemporal organization induced by oncogenes. Here, we apply superresolution microscopy in combination with image cross correlation spectroscopy to the U937-PR9 cell line, an in vitro model of acute promyelocytic leukemia that allows us to activate the expression of the PML-RARα oncogene and analyze its effects on the spatiotemporal organization of functional nuclear processes. More specifically, we perform Tau-stimulated emission depletion imaging, a superresolution technique based o

SUBMITTER: Cerutti E 

PROVIDER: S-EPMC9703036 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature