Culture-Associated DNA Methylation Changes Impact on Cellular Function of Human Intestinal Organoids.

ABSTRACT:

Background & aims

Human intestinal epithelial organoids (IEOs) are a powerful tool to model major aspects of intestinal development, health, and diseases because patient-derived cultures retain many features found in vivo. A necessary aspect of the organoid model is the requirement to expand cultures in vitro through several rounds of passaging. This is of concern because the passaging of cells has been shown to affect cell morphology, ploidy, and function.

Methods

Here, we analyzed 173 human IEO lines derived from the small and large bowel and examined the effect of culture duration on DNA methylation (DNAm). Furthermore, we tested the potential impact of DNAm changes on gene expression and cellular function.

Results

Our analyses show a reproducible effect of culture

SUBMITTER: Edgar RD 

PROVIDER: S-EPMC9703134 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature