From phenotypical investigation to RNA-sequencing for gene expression analysis: A workflow for single and pooled rare cells.

ABSTRACT: Combining phenotypical and molecular characterization of rare cells is challenging due to their scarcity and difficult handling. In oncology, circulating tumor cells (CTCs) are considered among the most important rare cell populations. Their phenotypic and molecular characterization is necessary to define the molecular mechanisms underlying their metastatic potential. Several approaches that require cell fixation make difficult downstream molecular investigations on RNA. Conversely, the DEPArray technology allows phenotypic analysis and handling of both fixed and unfixed cells, enabling a wider range of applications. Here, we describe an experimental workflow that allows the transcriptomic investigation of single and pooled OE33 cells undergone to DEPArray analysis and recovery. In additio

SUBMITTER: Rossi T 

PROVIDER: S-EPMC9703136 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature