Snapshots of urea-induced early structural changes and unfolding of an ankyrin repeat protein at atomic resolution.

ABSTRACT: Protein folding and unfolding is a complex process, underscored by the many proteotoxic diseases associated with misfolded proteins. Mapping pathways from a native structure to an unfolded protein or vice versa, identifying the intermediates, and defining the role of sequence and structure en route remain outstanding problems in the field. It is even more challenging to capture the events at atomistic resolution. X-ray diffraction has so far been used to understand how urea interacts with and unfolds two stable globular proteins. Here, we present the case study on PSMD10^Gankyrin , a prototype for a moderately stable, non-globular repeat protein, long and rigid, with its termini located at either end.   We define structural changes in the time dimension using low urea concentrati

SUBMITTER: Medur Gurushankar MS 

PROVIDER: S-EPMC9703593 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature