Combined transcriptome and proteome analysis of yak PASMCs under hypoxic and normoxic conditions.

ABSTRACT:

Background

Yaks are animals that have lived in plateau environments for generations. Yaks can adapt to the hypoxic plateau environment and also pass this adaptability on to the next generation. The lungs are the most important respiratory organs for mammals to adapt to their environment. Pulmonary artery smooth muscle cells play an important role in vascular remodeling under hypoxia, but the genetic mechanism underpinning the yak's ability to adapt to challenging plateau conditions is still unknown.

Methods

A tandem mass tag (TMT) proteomics study together with an RNA-seq transcriptome analysis were carried out on pulmonary artery smooth muscle cells (PASMCs) that had been grown for 72 hours in both normoxic (20% O2) and hypoxic (1% O2) environments. RNA and TP (total protei

SUBMITTER: Zhang L 

PROVIDER: S-EPMC9703989 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature