Deciphering the anti-filarial potential of bioactive compounds from Ocimum sanctum: a combined experimental and computational study.

ABSTRACT:

Context

The anthelminthic effect of Ocimum species (Lamiaceae) has been reported, however, its anti-filarial effect has not been explored to date.

Objective

This study evaluates the effect of Ocimum sanctum L. (OS) against lymphatic filarial parasites.

Material and methods

The ethanol extract of OS (EOS) leaves was tested for anti-filarial activity against Setaria cervi. Equal size and number (n = 10) of adult female S. cervi worms were incubated in 125, 250 or 375 μg/mL EOS extract for 6 h at 37 °C. The OS bioactive components were identified by UPLC-ESI-MS/MS and subjected to docking and molecular dynamics (MD) simulation against filarial antioxidant proteins.

Results

The EOS significantly inhibited the motility of adult fem

SUBMITTER: Mishra A 

PROVIDER: S-EPMC9704100 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature