High frequency of increased triclosan MIC among CC5 MRSA and risk of misclassification of the SCCmec into types.

ABSTRACT:

Background

Typing of staphylococcal cassette chromosome mec (SCCmec) elements is commonly used for studies on the molecular epidemiology of MRSA.

Objectives

To perform an investigation centred on uncovering the reasons for misclassification of MRSA clonal complex 5 (CC5) SCCmec type II clinical isolates in our laboratory.

Methods

MRSA isolates from CC5 were subjected to WGS and SCCmec typing.

Results

This investigation led to the discovery that the classification failure was due to an insertion of IS1272 carrying the fabI gene on a transposable element (TnSha1) that confers increased MIC to the biocide triclosan. Genomic analysis revealed that fabI was present in 25% of the CC5 MRSA isolates sampled. The frequency of TnSha1 in our collection was much higher tha

SUBMITTER: Viana AS 

PROVIDER: S-EPMC9704425 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature