A high-efficiency scar-free genome-editing toolkit for Acinetobacter baumannii.

ABSTRACT:

Background

The current mutagenesis tools for Acinetobacter baumannii leave selection markers or residual sequences behind, or involve tedious counterselection and screening steps. Furthermore, they are usually adapted for model strains, rather than for MDR clinical isolates.

Objectives

To develop a scar-free genome-editing tool suitable for chromosomal and plasmid modifications in MDR A. baumannii AB5075.

Methods

We prove the efficiency of our adapted genome-editing system by deleting the multidrug efflux pumps craA, cmlA5 and resistance island 2 (RI2), as well as curing plasmid p1AB5075, and combining these mutations. We then characterized the susceptibility of the mutants compared with the WT to different antibiotics (i.e. chloramphenicol, amikacin and tobramycin) b

SUBMITTER: de Dios R 

PROVIDER: S-EPMC9704439 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature