MiR-302d inhibits TGFB-induced EMT and promotes MET in primary human RPE cells

ABSTRACT:

Purpose

Transforming growth factor-beta (TGFB)-mediated epithelial-mesenchymal transition (EMT) plays a crucial role in the pathogenesis of retinal fibrosis, which is one of the leading causes of impaired vision. Current approaches to treating retinal fibrosis focus, among other things, on inhibiting the TGFB signaling pathway. Transient expression of microRNAs (miRNAs) is one way to inhibit the TGFB pathway post-transcriptionally. Our previous study identified the miRNA miR-302d as a regulator of multiple TGFB-related genes in ARPE-19 cells. To further explore its effect on primary cells, the effect of miR-302d on TGFB-induced EMT in primary human retinal pigment epithelium (hRPE) was investigated in vitro.

Methods

hRPE cells were extracted from patients receiving enucle

SUBMITTER: Hu X 

PROVIDER: S-EPMC9704570 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature