Vaccine protection by Cryptococcus neoformans Δsgl1 is mediated by γδ T cells via TLR2 signaling.

ABSTRACT: We previously reported that administration of Cryptococcus neoformans Δsgl1 mutant vaccine, accumulating sterylglucosides (SGs) and having normal capsule (GXM), protects mice from a subsequent infection even during CD4⁺ T cells deficiency, a condition commonly associated with cryptococcosis. Here, we studied the immune mechanism that confers host protection during CD4⁺T deficiency. Mice receiving Δsgl1 vaccine produce IFNγ and IL-17A during CD4⁺ T (or CD8⁺ T) deficiency, and protection was lost when either cytokine was neutralized. IFNγ and/or IL-17A are produced by γδ T cells, and mice lacking these cells are no longer protected. Interestingly, ex vivo γδ T cells are highly stimulated in producing IFNγ and/or IL-17A by Δsgl1 vaccine, but this pr

SUBMITTER: Normile TG 

PROVIDER: S-EPMC9705245 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature