Organotypic stromal cells impact endothelial cell transcriptome in 3D microvessel networks.

ABSTRACT: Endothelial cells line all major blood vessels and serve as integral regulators of many functions including vessel diameter, cellular trafficking, and transport of soluble mediators. Despite similar functions, the phenotype of endothelial cells is highly organ-specific, yet our understanding of the mechanisms leading to organ-level differentiation is incomplete. We generated 3D microvessel networks by combining a common naïve endothelial cell with six different stromal cells derived from the lung, skin, heart, bone marrow, pancreas, and pancreatic cancer. Single cell RNA-Seq analysis of the microvessel networks reveals five distinct endothelial cell populations, for which the relative proportion depends on the stromal cell population. Morphologic features of the organotypic vessel networks

SUBMITTER: Curtis MB 

PROVIDER: S-EPMC9705391 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature