A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems.

ABSTRACT: In vitro systems mimicking brain regions, brain organoids, are revolutionizing the neuroscience field. However, characterization of their electrical activity has remained a challenge as it requires readout at millisecond timescale in 3D at single-neuron resolution. While custom-built microscopes used with genetically encoded sensors are now opening this door, a full 3D characterization of organoid neural activity has not been performed yet, limited by the combined complexity of the optical and the biological system. Here, we introduce an accessible minimalistic light-sheet microscope to the neuroscience community. Designed as an add-on to a standard inverted microscope it can be assembled within one day. In contrast to existing simplistic setups, our platform is suited to record volumetric

SUBMITTER: Wysmolek PM 

PROVIDER: S-EPMC9705530 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature