Dataset Information

Characterization and Whole Genome Sequencing of Chromobacterium violaceum OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant.

ABSTRACT: This study reports a rare fatal case of Chromobacterium violeceum OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gene sequence measuring 1410 bp revealed 97% homology with C. violeceum. The bacterial genome composed of 64.87% of G + C content with total size of 4,681,202 bp. The genome annotation has 42 genes responsible for multidrug antibiotic resistance with the presence of Aminoglycoside-modifying enzymes (AAC (6')) that targets streptomycin and spectinomycin. Our findings corroborated the lethal effect of C. violeceum in a new host (elephant) that enriched scientific information on epidemiological picture and whole genome sequencing as well.

Supplementary information

The online version contains supplementary material available at 10.1007/s12088-022-01047-4.

SUBMITTER: Hota SR

PROVIDER: S-EPMC9705667 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature

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Characterization and Whole Genome Sequencing of <i>Chromobacterium violaceum</i> OUAT_2017: A Zoonotic Pathogen Found Fatal to a Wild Asiatic Elephant.

Hota Sourav R SR Padhi Soumesh K SK Pahari Avishek A Behera Bikash K BK Panda Biswaranjan B Mor Sunil K SK Singh Vikash K VK Goyal Sagar M SM Sahoo Niranjana N

Indian journal of microbiology 20221031 4

This study reports a rare fatal case of <i>Chromobacterium violeceum</i> OUAT_2017 strain infection in an Asiatic elephant calf in India. Necropsy revealed pus-filled nodules in liver, spleen, and lungs. Nutrient broth cultures of nodule content showed sediment of violet pigment whereas smooth, non-diffusible, violet-pigmented, homogeneous colonies appeared on nutrient agar. The organism was found to be non-haemolytic and resistant to 8 of the 24 antibiotics tested in vitro. Partial 16S rRNA gen ...[more]

PMID: 36458219

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