Protein painting reveals pervasive remodeling of conserved proteostasis machinery in response to pharmacological stimuli.

ABSTRACT: The correct spatio-temporal organization of the proteome is essential for cellular homeostasis. However, a detailed mechanistic understanding of this organization and how it is altered in response to external stimuli in the intact cellular environment is as-yet unrealized. 'Protein painting methods provide a means to address this gap in knowledge by monitoring the conformational status of proteins within cells at the proteome-wide scale. Here, we demonstrate the ability of a protein painting method employing tetraphenylethene maleimide (TPE-MI) to reveal proteome network remodeling in whole cells in response to a cohort of commonly used pharmacological stimuli of varying specificity. We report specific, albeit heterogeneous, responses to individual stimuli that coalesce on a conserved set

SUBMITTER: Cox D 

PROVIDER: S-EPMC9705707 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature