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Immobilization of GH78 α-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe₃O₄-SiO₂-NH₂-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin.

ABSTRACT: To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe₃O₄-SiO₂-NH₂-Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe₃O₄-NH₂ with tetraethyl silicate (TEOS), microcrystalline cellulose and zinc nitrate hexahydrate. FSNcZ8 displayed better magnetic stability and higher-temperature stability than unmodified Fe₃O₄-NH₂ (FN), and it was used to adsorb and immobilize TpeRha from Thermotoga petrophilea 13995. As for properties, FSNcZ8-TpeRha showed optimal reaction temperature and pH of 90°C and 5.0, while its highest activity approached 714 U/g. In addition,

SUBMITTER: Xu J

PROVIDER: S-EPMC9705872 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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Immobilization of GH78 α-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe₃O₄-SiO₂-NH₂-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin.

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Immobilization of GH78 α-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe3O4-SiO2-NH2-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin.

OmicsDI is part of the ELIXIR infrastructure

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Immobilization of GH78 α-L-Rhamnosidase from Thermotoga petrophilea with High-Temperature-Resistant Magnetic Particles Fe₃O₄-SiO₂-NH₂-Cellu-ZIF8 and Its Application in the Production of Prunin Form Naringin.