Purification and Characterization of a Thrombolytic Enzyme Produced by a New Strain of Bacillus subtil.

ABSTRACT: Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. This paper reports on a new enzyme capable of degrading blood clots directly without impairing blood coagulation. This enzyme is also non-cytotoxic and constitutes an alternative to other thrombolytic enzymes known to cause undesired side effects. Twenty-four Bacillus isolates were screened for production of fibrinolytic enzymes using a fibrin agar plate. Based on produced activity, isolate S127e was selected and identified as B. subtilis using the 16S rDNA gene sequence. This strain is of biotechnological interest for producing high fibrinolytic yield and consequently has potential in the industrial field. The purified fibrinolytic enzyme has a molecu

SUBMITTER: Frias J 

PROVIDER: S-EPMC9705946 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature