Immunoaffinity-based mass spectrometric characterization of immunoreactive proteins of Salmonella Typhi.

ABSTRACT: Salmonella Typhi, a human-restricted Gram negative enterobacteriaceae, is the causative agent of typhoid fever in human being. The available serodiagnostic tools for the diagnosis of typhoid fever lack sensitivity and/or specificity. This study aimed to identify the immunoreactive proteins of S. Typhi that could help to develop improved diagnostic tools. Here, we performed immunoaffinity-based proteomic approach that uses charged columns to retrieve IgG and IgM antibodies from the plasma of typhoid patients followed by capture of S. Typhi proteins. These proteins were then characterized by mass spectrometry and bioinformatics tools. Using this approach, we identified 28 immunoreactive proteins of S. Typhi, in which 14 proteins were captured by IgG charged column

SUBMITTER: Safi AUR 

PROVIDER: S-EPMC9706174 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature