Antibodies Produced Toward Recombinant RBD and Nucleocapsid Neutralize SARS-COV-2.

ABSTRACT:

Background

The highly contagious SARS-COV-2 virus spread rapidly from China and formed a global pandemic. The virus has infected over 509 million people worldwide and killed about 6.32 million up to date. Up on invasion, the Receptor Binding Domain (RBD) of Spike protein plays a crucial role in the entry of the virus into the host cell. The virus N protein is another protein that has a critical role for genome packaging.

Methods

As bioinformatics approaches, the cassette design, codon adaptation, and protein stability were investigated in this study. Synthetic genes of RBD and N were cloned separately in pET28a + expression vector. They were transferred into Escherichia coli (E. coli) BL21 DE3 host cell, and expression of recombinant proteins was induced

SUBMITTER: Rezaei A 

PROVIDER: S-EPMC9706246 | biostudies-literature | 2022 Oct-Dec

REPOSITORIES: biostudies-literature