Short PolyA RNA Homopolymers Undergo Mg²⁺-Mediated Kinetically Arrested Condensation.

ABSTRACT: RNA-RNA interactions have increasingly been recognized for their potential to shape the mesoscale properties of biomolecular condensates, influencing morphology, organization, and material state through networking interactions. While most studies have focused on networking via Watson-Crick base pairing interactions, previous work has suggested a potential for noncanonical RNA-RNA interactions to also give rise to condensation and alter overall material state. Here, we test the phase separation of short polyA RNA (polyrA) homopolymers. We discover and characterize the potential for short polyrA sequences to form RNA condensates at lower Mg²⁺ concentrations than previously observed, which appear as internally arrested droplets with slow polyrA diffusion despite continued fusion. O

SUBMITTER: Tom JKA 

PROVIDER: S-EPMC9706566 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature