Hydroxyurea ameliorates atherosclerosis in ApoE^-/- mice by potentially modulating Niemann-Pick C1-like 1 protein through the gut microbiota.

ABSTRACT: Rationale: The efficacy and mechanism of hydroxyurea in the treatment of atherosclerosis have rarely been reported. The goal of this study was to investigate the efficacy of hydroxyurea in high-fat diet-fed ApoE^-/- mice against atherosclerosis and examine the possible mechanism underlying treatment outcomes. Methods: ApoE^-/- mice were fed a high-fat diet for 1 month and then administered hydroxyurea by gavage continuously for 2 months. Aortic root hematoxylin-eosin (H&E) staining and oil red O staining were used to verify the efficacy of hydroxyurea; biochemical methods and ELISA were used to detect changes in relevant metabolites in serum. 16S rRNA was used to detect composition changes in the intestinal bacterial community of animals after treatment wi

SUBMITTER: Yang XY 

PROVIDER: S-EPMC9706578 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature