Multiomic analysis for optimization of combined focal and immunotherapy protocols in murine pancreatic cancer.

ABSTRACT: Background: Although combination immunotherapies incorporating local and systemic components have shown promising results in treating solid tumors, varied tumor microenvironments (TMEs) can impact immunotherapeutic efficacy. Method: We designed and evaluated treatment strategies for breast and pancreatic cancer combining magnetic resonance-guided focused ultrasound (MRgFUS) ablation and antibody therapies. With a combination of single-cell sequencing, spectral flow cytometry, and histological analyses, we profiled an immune-suppressed KPC (Kras^+/LSL-G12D; Trp53^+/LSL-R172H; Pdx1-Cre) pancreatic adenocarcinoma (MT4) model and a dense epithelial neu deletion (NDL) HER2⁺ mammary adenocarcinoma model with a greater fraction of lymphocytes, natural

SUBMITTER: Wang J 

PROVIDER: S-EPMC9706583 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature