Self-assembly of X-shaped antibody to combine the activity of IgG and IgA for enhanced tumor killing.

ABSTRACT: Rationale: IgA can induce activation of neutrophils which are the most abundant cell type in blood, but the development of IgA as therapeutic has been confounded by its short half-life and a weak ability to recruit NK cells as effector cells. Therefore, we generated an X-shaped antibody (X-body) based on the principle of molecular self-assembly that combines the activities of both IgG and IgA, which can effectively recruit and activate NK cells, macrophages, and neutrophils to kill tumor cells. Methods: X-body was generated by using a self-assembly strategy. The affinity of the X-body with the antigen and Fc receptors was tested by surface plasmon resonance. The shape of X-body was examined using negative staining transmission electron microscopy. The tumor cell killing activ

SUBMITTER: Liang Y 

PROVIDER: S-EPMC9706586 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature