Phase Reconstruction of Low-Energy Electron Holograms of Individual Proteins.

ABSTRACT: Low-energy electron holography (LEEH) is one of the few techniques capable of imaging large and complex three-dimensional molecules, such as proteins, on the single-molecule level at subnanometer resolution. During the imaging process, the structural information about the object is recorded both in the amplitude and in the phase of the hologram. In low-energy electron holography imaging of proteins, the object's amplitude distribution, which directly reveals molecular size and shape on the single-molecule level, can be retrieved via a one-step reconstruction process. However, such a one-step reconstruction routine cannot directly recover the phase information encoded in the hologram. In order to extract the full information about the imaged molecules, we thus implemented an iterative phase

SUBMITTER: Ochner H 

PROVIDER: S-EPMC9706659 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature