Targeting Apollo-NADP⁺ to Image NADPH Generation in Pancreatic Beta-Cell Organelles.

ABSTRACT: NADPH/NADP⁺ redox state supports numerous reactions related to cell growth and survival; yet the full impact is difficult to appreciate due to organelle compartmentalization of NADPH and NADP⁺. To study glucose-stimulated NADPH production in pancreatic beta-cell organelles, we targeted the Apollo-NADP⁺ sensor by first selecting the most pH-stable version of the single-color sensor. We subsequently targeted mTurquoise2-Apollo-NADP⁺ to various organelles and confirmed activity in the cytoplasm, mitochondrial matrix, nucleus, and peroxisome. Finally, we measured the glucose- and glutamine-stimulated NADPH responses by single- and dual-color imaging of the targeted sensors. Overall, we developed multiple organelle-targeted Apollo-NADP⁺ sen

SUBMITTER: Chang HH 

PROVIDER: S-EPMC9706804 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature