Lncap-AI prostate cancer cell line establishment by Flutamide and androgen-free environment to promote cell adherent.

ABSTRACT:

Background

To establish castration-resistant prostate cancer (CRPC) - Lncap androgen-independent (AI) cell line from Lncap androgen-dependent (AD) cell line, and explore the different molecular biological between these two cell lines.

Methods

The Lncap-AD cell line was cultured and passaged 60 times over 16 months. The morphology of the Lncap-AI cell line was observed. AR levels identification were detected in qRT-PCR and Western Blot assay. CCK-8, EdU assay, wound healing assay and cell adhesion assays were used to observe the ability of proliferation, migration, and adhesion. SEM and TEM were used to observe microculture structure. At last, the PSA secrete ability was evaluated by Elisa assay.

Results

The Lncap-AD cell line was cultured and passaged 60 times over 16

SUBMITTER: Wang H 

PROVIDER: S-EPMC9706963 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature