Identification of common and distinct origins of human serum and breastmilk IgA1 by mass spectrometry-based clonal profiling.

ABSTRACT: The most abundant immunoglobulin present in the human body is IgA. It has the highest concentrations at the mucosal lining and in biofluids such as milk and is the second most abundant class of antibodies in serum. We assessed the structural diversity and clonal repertoire of IgA1-containing molecular assemblies longitudinally in human serum and milk from three donors using a mass spectrometry-based approach. IgA-containing molecules purified from serum or milk were assessed by the release and subsequent analysis of their Fab fragments. Our data revealed that serum IgA1 consists of two distinct structural populations, namely monomeric IgA1 (∼80%) and dimeric joining (J-) chain coupled IgA1 (∼20%). Also, we confirmed that IgA1 in milk is present solely as secretory (S)IgA, consisting of two

SUBMITTER: Dingess KA 

PROVIDER: S-EPMC9707141 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature