Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein.

ABSTRACT: We recently identified a CD63-interacting protein to understand the role of CD63 in virion production of the human immunodeficiency virus type 1, and we have found that Rab3a forms a complex with CD63. In this study, we analysed the effect of Rab3a on virion production of the murine leukaemia virus (MLV), which is another member of the retrovirus family. We found that Rab3a silencing induced lysosomal degradation of the MLV Gag protein, and recovery of the Rab3a expression restored the level of the Gag protein through a complex formation of MLV Gag and Rab3a, indicating that Rab3a is required for MLV Gag protein expression. In contrast, CD63 silencing decreased the infectivity of released virions but had no effect on virion production, indicating that CD63 facilitates the infectivity of re

SUBMITTER: Izumida M 

PROVIDER: S-EPMC9707528 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature