Optimization of Lung Surfactant Coating of siRNA Polyplexes for Pulmonary Delivery

ABSTRACT:

Purpose

The aim of this study was to understand how coating with a pulmonary surfactant, namely Alveofact, affects the physicochemical parameters as well as in vitro behavior of polyethylenimine (PEI) polyplexes for pulmonary siRNA delivery.

Methods

Alveofact-coated polyplexes were prepared at different Alveofact:PEI coating ratios and analyzed in terms of size, PDI and zeta potential as well as morphology by transmission electron microscopy. The biological behavior was evaluated in a lung epithelial cell line regarding cell viability, cellular uptake via flow cytometry and gene downregulation by qRT-PCR. Furthermore, a 3D ALI culture model was established to test the mucus diffusion and cellular uptake by confocal microscopy as well as gene silencing activity by qRT-PCR.

SUBMITTER: Baldassi D 

PROVIDER: S-EPMC9708138 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature