Association of fecal and serum microRNA profiles with gastrointestinal cancer and chronic inflammatory enteropathy in dogs.

ABSTRACT:

Background

Reliable biomarkers to differentiate gastrointestinal cancer (GIC) from chronic inflammatory enteropathy (CIE) in dogs are needed. Fecal and serum microRNAs (miRNAs) have been proposed as diagnostic and prognostic markers of GI disease in humans and dogs.

Hypothesis/objectives

Dogs with GIC have fecal and serum miRNA profiles that differ from those of dogs with CIE.

Aims

(a) identify miRNAs that differentiate GIC from CIE, (b) use high-throughput reverse transcription quantitative real-time PCR (RT-qPCR) to establish fecal and serum miRNA panels to distinguish GIC from CIE in dogs.

Animals

Twenty-four dogs with GIC, 10 dogs with CIE, and 10 healthy dogs, all client-owned.

Methods

An international multicenter observational prospective case-cont

SUBMITTER: Lyngby JG 

PROVIDER: S-EPMC9708456 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature