Single-cell transcriptome analysis of tumor immune microenvironment characteristics in colorectal cancer liver metastasis.

ABSTRACT:

Background

Liver metastasis is the leading cause of death in colorectal cancer (CRC) patients, and the precise mechanisms remain unclear. In this study, single-cell RNA sequencing (scRNA-seq) was used to analyze the cellular and molecular heterogeneity between CRC primary lesion and corresponding liver metastasis, and to clarify the characteristics of the tumor microenvironment (TME) in synchronous liver metastasis of CRC.

Methods

A case of microsatellite stable (MSS) sigmoid carcinoma with synchronous liver metastasis was selected, and tissues from the primary tumor and the liver metastasis were collected for scRNA-seq. The EdgeR package software was used to identify the differentially expressed genes between cells. Gene Set Enrichment Analysis (GSEA) was performed and the

SUBMITTER: Geng Y 

PROVIDER: S-EPMC9708492 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature