The evolution of plant proton pump regulation via the R domain may have facilitated plant terrestrialization.

ABSTRACT: Plasma membrane (PM) H⁺-ATPases are the electrogenic proton pumps that export H⁺ from plant and fungal cells to acidify the surroundings and generate a membrane potential. Plant PM H⁺-ATPases are equipped with a C‑terminal autoinhibitory regulatory (R) domain of about 100 amino acid residues, which could not be identified in the PM H⁺-ATPases of green algae but appeared fully developed in immediate streptophyte algal predecessors of land plants. To explore the physiological significance of this domain, we created in vivo C-terminal truncations of autoinhibited PM H⁺‑ATPase2 (AHA2), one of the two major isoforms in the land plant Arabidopsis thaliana. As more residues were deleted, the mutant plants became progressively more efficient i

SUBMITTER: Steger A 

PROVIDER: S-EPMC9708826 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature