Mechano-growth factor E-domain modulates cardiac contractile function through 14-3-3 protein interactomes.

ABSTRACT: In the heart, alternative splicing of the igf-I gene produces two isoforms: IGF-IEa and IGF-IEc, (Mechano-growth factor, MGF). The sequence divergence between their E-domain regions suggests differential isoform function. To define the biological actions of MGF's E-domain, we performed in silico analysis of the unique C-terminal sequence and identified a phosphorylation consensus site residing within a putative 14-3-3 binding motif. To test the functional significance of Ser 18 phosphorylation, phospho-mimetic (S/E¹⁸) and phospho-null (S/A¹⁸) peptides were delivered to mice at different doses for 2 weeks. Cardiovascular function was measured using echocardiography and a pressure-volume catheter. At the lowest (2.25 mg/kg/day) and highest (9 mg/kg/day) do

SUBMITTER: Solis C 

PROVIDER: S-EPMC9709209 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature