Optimal combinations of fluorescent vessel labeling and tissue clearing methods for three-dimensional visualization of vasculature.

ABSTRACT:

Significance

Visualization of intact vasculatures is crucial to understanding the pathogeneses of different neurological and vascular diseases. Although various fluorescent vessel labeling methods have been used in combination with tissue clearing for three-dimensional (3D) visualization of different vascular networks, little has been done to quantify the labeling effect of each vessel labeling routine, as well as their applicability alongside various clearing protocols, making it difficult to select an optimal combination for finely constructing different vasculatures. Therefore, it is necessary to systematically assess the overall performance of these common vessel labeling methods combined with different tissue-clearing protocols.

Aim

A comprehensive evaluation of the lab

SUBMITTER: Zhu J 

PROVIDER: S-EPMC9709454 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature