An optimized approach and inflation media for obtaining complimentary mass spectrometry-based omics data from human lung tissue.

ABSTRACT: Human disease states are biomolecularly multifaceted and can span across phenotypic states, therefore it is important to understand diseases on all levels, across cell types, and within and across microanatomical tissue compartments. To obtain an accurate and representative view of the molecular landscape within human lungs, this fragile tissue must be inflated and embedded to maintain spatial fidelity of the location of molecules and minimize molecular degradation for molecular imaging experiments. Here, we evaluated agarose inflation and carboxymethyl cellulose embedding media and determined effective tissue preparation protocols for performing bulk and spatial mass spectrometry-based omics measurements. Mass spectrometry imaging methods were optimized to boost the number of annotatable

SUBMITTER: Lukowski JK 

PROVIDER: S-EPMC9709465 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature