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Optical Nanosensor for Intracellular and Intracranial Detection of Amyloid-Beta.

ABSTRACT: Amyloid-beta (Aβ) deposition occurs in the early stages of Alzheimer's disease (AD), but the early detection of Aβ is a persistent challenge. Herein, we engineered a near-infrared optical nanosensor capable of detecting Aβ intracellularly in live cells and intracranially in vivo. The sensor is composed of single-walled carbon nanotubes functionalized with Aβ wherein Aβ-Aβ interactions drive the response. We found that the Aβ nanosensors selectively responded to Aβ via solvatochromic modulation of the near-infrared emission of the nanotube. The sensor tracked Aβ accumulation in live cells and, upon intracranial administration in a genetic model of AD, signaled distinct responses in aged mice. This technology enables the interrogation of molecular mechanisms underlying Aβ neurotoxicity in the development of AD in living systems.

SUBMITTER: Antman-Passig M

PROVIDER: S-EPMC9710299 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Optical Nanosensor for Intracellular and Intracranial Detection of Amyloid-Beta.

Antman-Passig Merav M Wong Eitan E Frost Georgia R GR Cupo Christian C Shah Janki J Agustinus Albert A Chen Ziyu Z Mancinelli Chiara C Kamel Maikel M Li Thomas T Jonas Lauren A LA Li Yue-Ming YM Heller Daniel A DA

ACS nano 20220414 5

Amyloid-beta (Aβ) deposition occurs in the early stages of Alzheimer's disease (AD), but the early detection of Aβ is a persistent challenge. Herein, we engineered a near-infrared optical nanosensor capable of detecting Aβ intracellularly in live cells and intracranially in vivo. The sensor is composed of single-walled carbon nanotubes functionalized with Aβ wherein Aβ-Aβ interactions drive the response. We found that the Aβ nanosensors selectively responded to Aβ via solvatochromic modulation o ...[more]

PMID: 35420796

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