SangeR: the high-throughput Sanger sequencing analysis pipeline

ABSTRACT: Abstract

Summary

In the era of next generation sequencing and beyond, the Sanger technique is still widely used for variant verification of inconclusive or ambiguous high-throughput sequencing results or as a low-cost molecular genetical analysis tool for single targets in many fields of study. Many analysis steps need time-consuming manual intervention. Therefore, we present here a pipeline-capable high-throughput solution with an optional Shiny web interface, that provides a binary mutation decision of hotspots together with plotted chromatograms including annotations via flat files.

Availability and implementation

SangeR is freely available at https://github.com/Neuropathology-Giessen/SangeR and https://hub.docker.com/repository/docker/kaischmid/sange_r

Contact

SUBMITTER: Schmid K 

PROVIDER: S-EPMC9710597 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature