App-SpaM: phylogenetic placement of short reads without sequence alignment.

ABSTRACT:

Motivation

Phylogenetic placement is the task of placing a query sequence of unknown taxonomic origin into a given phylogenetic tree of a set of reference sequences. A major field of application of such methods is, for example, the taxonomic identification of reads in metabarcoding or metagenomic studies. Several approaches to phylogenetic placement have been proposed in recent years. The most accurate of them requires a multiple sequence alignment of the references as input. However, calculating multiple alignments is not only time-consuming but also limits the applicability of these approaches.

Results

Herein, we propose Alignment-free phylogenetic placement algorithm based on Spaced-word Matches (App-SpaM), an efficient algorithm for the phylogenetic placeme

SUBMITTER: Blanke M 

PROVIDER: S-EPMC9710606 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature