PoET: automated approach for measuring pore edge tension in giant unilamellar vesicles.

ABSTRACT:

Motivation

A reliable characterization of the membrane pore edge tension of single giant unilamellar vesicles (GUVs) requires the measurement of micrometer sized pores in hundreds to thousands of images. When manually performed, this procedure has shown to be extremely time-consuming and to generate inconsistent results among different users and imaging systems. A user-friendly software for such analysis allowing quick processing and generation of reproducible data had not yet been reported.

Results

We have developed a software (PoET) for automatic pore edge tension measurements on GUVs. The required image processing steps and the characterization of the pore dynamics are performed automatically within the software and its use allowed for a 30-fold reduction in the analysis

SUBMITTER: Leomil FSC 

PROVIDER: S-EPMC9710609 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature