LRez: a C++ API and toolkit for analyzing and managing Linked-Reads data.

ABSTRACT:

Motivation

Linked-Reads technologies combine both the high quality and low cost of short-reads sequencing and long-range information, through the use of barcodes tagging reads which originate from a common long DNA molecule. This technology has been employed in a broad range of applications including genome assembly, phasing and scaffolding, as well as structural variant calling. However, to date, no tool or API dedicated to the manipulation of Linked-Reads data exist.

Results

We introduce LRez, a C++ API and toolkit that allows easy management of Linked-Reads data. LRez includes various functionalities, for computing numbers of common barcodes between genomic regions, extracting barcodes from BAM files, as well as indexing and querying BAM, FASTQ and gzipped FASTQ files to

SUBMITTER: Morisse P 

PROVIDER: S-EPMC9710615 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature