Xeno-Free Integrated Platform for Robust Production of Cardiomyocyte Sheets from hiPSCs.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) can be efficiently differentiated into cardiomyocytes (CMs), which can be used for cardiac disease modeling, for drug screening, and to regenerate damaged myocardium. Implementation of xeno-free culture systems is essential to fully explore the potential of these cells. However, differentiation using xeno-free adhesion matrices often results in low CM yields and lack of functional CM sheets, capable of enduring additional maturation stages. Here, we established a xeno-free CM differentiation platform using TeSR/Synthemax, including a replating step and integrated with two versatile purification/enrichment metabolic approaches. Results showed that the replating step was essential to reestablish a fully integrated, closely-knit CM sheet. In addit

SUBMITTER: Dias TP 

PROVIDER: S-EPMC9712013 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature