Single-Cell Analysis Reveals the Range of Transcriptional States of Circulating Human Neutrophils.

ABSTRACT: Neutrophils are the most abundant leukocytes in human blood and are essential components of innate immunity. Until recently, neutrophils were considered homogeneous and transcriptionally inactive cells, but both concepts are being challenged. Single-cell RNA sequencing (scRNA-seq) offers an unbiased view of cells along a continuum of transcriptional states. However, the use of scRNA-seq to characterize neutrophils has proven technically difficult, explaining in part the paucity of published single-cell data on neutrophils. We have found that modifications to the data analysis pipeline, rather than to the existing scRNA-seq chemistries, can significantly increase the detection of human neutrophils in scRNA-seq. We have then applied a modified pipeline to the study of human peripheral blood

SUBMITTER: Wigerblad G 

PROVIDER: S-EPMC9712146 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature