A PCR-based method for the diagnosis of Enterobius vermicularis in stool samples, specifically designed for clinical application.

ABSTRACT:

Background

Enterobius vermicularis (E. vermicularis) is a nematode that infects up to 200 million people worldwide, despite effective medications being available. Conventional diagnostic tests are hindered by low sensitivity and poor patient compliance. Furthermore, no biomolecular techniques are available for clinical application. The aim of this study was to develop a procedure specifically designed for clinical application to detect E. vermicularis by means of PCR.

Materials and methods

Two subject groups were taken into account: a group of 27 infected patients and a control group of 27 healthy subjects. A nested-PCR was performed on fecal samples to detect E. vermicularis. Due to the intrinsic difficulties of the fecal matrix, several counterm

SUBMITTER: Ummarino A 

PROVIDER: S-EPMC9712443 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature