Deciphering the heterogeneity of the Lyve1⁺ perivascular macrophages in the mouse brain.

ABSTRACT: Perivascular macrophages (pvMs) are associated with cerebral vasculature and mediate brain drainage and immune regulation. Here, using reporter mouse models, whole brain and section immunofluorescence, flow cytometry, and single cell RNA sequencing, besides the Lyve1⁺F4/80⁺CD206⁺CX3CR1⁺ pvMs, we identify a CX3CR1^- pvM population that shares phagocytic functions and location. Furthermore, the brain parenchyma vasculature mostly hosts Lyve1⁺MHCII^- pvMs with low to intermediate CD45 expression. Using the double Cx3cr1^GFP x Cx3cr1-Cre;Rosa^tdT reporter mice for finer mapping of the lineages, we establish that CD45^lowCX3CR1^- pvMs are derived from CX3CR1⁺ precursors

SUBMITTER: Siret C 

PROVIDER: S-EPMC9712536 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature