Spatially resolved whole transcriptome profiling in human and mouse tissue using Digital Spatial Profiling.

ABSTRACT: Emerging spatial profiling technology has enabled high-plex molecular profiling in biological tissues, preserving the spatial and morphological context of gene expression. Here, we describe expanding the chemistry for the Digital Spatial Profiling platform to quantify whole transcriptomes in human and mouse tissues using a wide range of spatial profiling strategies and sample types. We designed multiplexed in situ hybridization probes targeting the protein-coding genes of the human and mouse transcriptomes, referred to as the human or mouse Whole Transcriptome Atlas (WTA). Human and mouse WTAs were validated in cell lines for concordance with orthogonal gene expression profiling methods in regions ranging from ∼10-500 cells. By benchmarking against bulk RNA-seq and fluorescence in situ hyb

SUBMITTER: Zimmerman SM 

PROVIDER: S-EPMC9712633 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature