Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks.

ABSTRACT:

Background

Sitting at the interface of gene expression and host-pathogen interaction, polymerase associated factor 1 complex (PAF1C) is a rising player in the innate immune response. The complex localizes to the nucleus and associates with chromatin to modulate RNA polymerase II (RNAPII) elongation of gene transcripts. Performing this function at both proximal and distal regulatory elements, PAF1C interacts with many host factors across such sites, along with several microbial proteins during infection. Therefore, translating the ubiquity of PAF1C into specific impacts on immune gene expression remains especially relevant.

Results

Advancing past work, we treat PAF1 knockout cells with a slate of immune stimuli to identify key trends in PAF1-dependent gene expression with bro

SUBMITTER: Kenaston MW 

PROVIDER: S-EPMC9713194 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature